Part:BBa_K4235032

LIC Primer (insert 3')

This is the reverse primer sequence used to amplify the insert BBa_K4235000 and create 3' overhangs for the consecutive LIC reaction.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 16
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 16
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 16
  
• 1000
  COMPATIBLE WITH RFC[1000]